| Congresso Brasileiro de Microbiologia 2023 | Resumo: 217-1 | ||||
Resumo:Propolis is a combination of wax, bee saliva and resinous compounds that bees collect from vegetation. Tubuna (Scaptotrigona bipunctata) and Borá (Tetragonisca angustula) are among the native bee propolis species found in the Brazilian biome. Among the chemical components in propolis extracts, antimicrobial activities attest to bacterial resistance to available antimicrobials with the possibility of therapeutic innovations and new antimicrobial drugs. The 2 samples of propolis extracts (PE) were obtained from meliponary farms: 1 EP from Tubuna in Rio Grande do Sul and 1 EP from Borá from the Federal District. The antibacterial activity of EP was verified by Determination of the Minimum Bactericidal Concentration (MBC). The antibacterial activity of the PE was verified by Determination of the Minimum Bactericidal Concentration (MBC). The inocula used were bacterial strains: Staphylococcus aureus ATCC 25923, Streptococcus mutans ATCC 25175 (Gram-positive), and Klebsiella pneumoniae ATCC BAA-1706 and Salmonella enterica ATCC 14028 (Gram-negative). The antioxidant activity of PE was evaluated through the reduction of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals. Tests were performed in triplicate and results expressed as mean in mM/mg TEAC (Trolox Equivalent Antioxidant Capacity). All EP in this study showed bactericidal activity against Gram-positive and Gram-negative bacteria tested. Regarding Gram-positive bacteria, MBC values of Tubuna EP were 0.5 mg/mL for S. aureus and 0.8 mg/mL for S. mutans. And the EP Borá presented MBC values of 0.08 mg/mL for S. aureus and 0.06 mg/mL for S. mutans. With the Gram-negative bacteria, EP Tubuna and Borá, they obtained the same MBC value of 0.3 mg/mL for K. pneumoniae. Tubuna EP showed CBM of 0.5 mg/mL for S. enterica. EP Borá presented CBM of 0.20 mg/mL for S. enterica. Regarding the antioxidant activity, EP from Borá showed higher antioxidant activity than EP from Tubuna. For Borá PE, the results ranged from 12.73 to 424.02 mM/mg TEAC for ABTS and from 36.41 to 162.53 mM/mg TEAC for DPPH. And for Tubuna PE, the results ranged from 0.33 to 0.54 mM/mg TEAC for ABTS and from 0.12 to 0.47 mM/mg TEAC for DPPH. In view of this, the extracts of Tubuna and Borá showed bactericidal activity against Gram-positive and Gram-negative bacteria, as well as the extract of Borá in oxidizing activity, therefore, the activities positively enable the development of new antimicrobial therapies with extracts of propolis from stingless bees native to Brazil.
Development Agency: This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. Palavras-chave: Anti-Infective Agents, Antioxidants, Gram-Negative Bacteria, Gram-Positive Bacteria, Propolis Agência de fomento:This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. | |||||